Aug 16, Tuesday

10:00 – 16:00  Registration (Lobby of Life Science Building)

12:30 – 13:45 Lunch (OEC Hostel III Dining Hall)

14:00    Opening by Sándor Damjanovich and György Vámosi(LSB F008-009)

14:20 – 15:10   Robert M. Clegg (U. Illinois, Urbana-Champaign, US): The life in a normal day (nanoseconds) of an excited fluorescent molecule

15:20 – 16:10   György Vereb (U. Debrecen, H): Don’t fret over FRET

16:20 – 16:40Coffee break

16:40 – 17:30   Jörg Langowski (DKFZ, Heidelberg, D): FCS and FCCS – introduction and applications in biology


17:40 – 18:30   Péter Nagy (U. Debrecen, H): Molecular interactions investigated by quantitative colocalization measurements and number & brightness analysis

– 19:30   Stefan Terjung (EMBL, Heidelberg, D): FRAP tutorial

19:30     Dinner (LSB lobby)

Aug 17, Wednesday

9:00 – 12:30     Practice

12:30 – 13:45 Lunch (OEC Hostel III Dining Hall)

14:00 – 16:30   Evaluation

16:30 – 17:00  Coffee Break

17:00      Katalin Tóth (DKFZ, Heidelberg, D): Structure, stability and diversity of nucleosomes studied by single pair FRET

17:40      György Vámosi (U. Debrecen, H): Interactions and function of membrane receptors and transcription factors studied by FRET and FCS

18:20      Robert M. Clegg (U. Illinois, Urbana-Champaign, US): Chasing Fluorescence Lifetimes in Complex Biological Systems.  What Can Fluorescence Lifetime Imaging Microscopy (FLIM) tell us?

19:30      Dinner (OEC Hostel III Dining Hall)

Aug 18, Thursday

9:00 – 12:30     Practice

12:30 – 13:45 Lunch (OEC Hostel III Dining Hall)

14:00 – 16:30   Evaluation

16:30 – 17:00 Coffee Break

17:00      Rainer Pepperkok (EMBL, Heidelberg, D): Studying the Coupling of COPII Vesicle Formation  to Microtubule-Mediated Transport by FRAP and Mathematcial Modelling

17:40      Claus Seidel (U. Düsseldorf, D): Multiparameter Fluorescence Image Spectroscopy (MFIS) to study molecular interactions I

18:45    Poster viewing with wine and cheese, 20:00-  dinner (LSB lobby)

Aug 19, Friday

9:00 – 12:30     Practice

12:30 – 13:45 Lunch (OEC Hostel III Dining Hall)

14:00 – 17:00   Practice

17:00 – 17:30Coffee Break

17:30      Claus Seidel (U. Düsseldorf, D): Multiparameter Fluorescence Image Spectroscopy (MFIS) to study molecular interactions II

18:10      Jerker Widengren (KTH Stockholm, S): Fast molecular dynamic processes monitored by fluorescence correlation spectroscopy

19:30     Dinner at Paripa Csárda (buses leave from Life Science Building)

Aug 20, Saturday

Viewing Flower Carnival (from the bleachers at Egyetem sugárút)

13:00 – 14:00 Lunch (OEC Hostel III Dining Hall)

14:30 - 16:30   Evaluation of Aug 19 morning practice

16:30 – 17:00Coffee break

17:00 - 19:00   Evaluation of Aug 19 afternoon practice

19:30      Dinner (OEC Hostel III Dining Hall)

Aug 21, Sunday

9:00 – 12:30     Practice

12:30 – 13:30 Lunch (OEC Hostel III Dining Hall)

13:30 – 15:30 Evaluation

16:00 – 17:00 Discussion of the labs presented by participants

17:00  17:30 Coffee break

17:30      Giuseppe Chirico (U. Milan, I): Fluorescence Correlation Spectroscopy methods for in vitro and in vivo experiments

18:10      János Szöllősi (U. Debrecen, H): Novel FRET approaches for studying interactions of membrane molecules

18:50      Malte Renz (NIH, Bethesda, US): Single-Molecule Counting with PALM

19:50      Dinner (OEC Hostel III Dining Hall)


Aug 22, Monday, EBSA Satellite Meeting on “FCS in Cell Biology”

Session 1

9:00       Opening

9:10        Yves Engelborghs (U. Leuven, B): Protein diffusion and interactions in vitro and in the living cell as studied by Fluorescence Correlation Spectroscopy

9:40        Roland Brock(U. Nijmegen, NL): Quantitative Analysis of Signaling Proteins and Complex Formation in T Cell Signal Transduction

10:10      Thomas Weidemann(U. Dresden, D): Advanced concepts of fluorescence correlation spectroscopy to study molecular interactions in cytokine receptor signaling

10:40 - 11.00      Coffee break

Session 2

11:00      Jerker Widengren(KTH, Stockholm, S): Transient state monitoring – approaches and possible benefits for biomolecular studies

11:30      Markus Sauer(U. Würzburg, D): Super-Resolution Imaging by dSTORM

12:00      Giuseppe Chirico (U. Milan, I): Diffusion-Photodynamics coupling in FCS studies of photo-switchable GFP proteins

12.30-13:30       Lunch (LSB Lobby)

13:30-14:50     Poster session (LSB Lobby)


Session 3

14:50      Jörg Langowski(DKFZ, Heidelberg, D): Single Plane Illumination Fluorescence Correlation Spectroscopy (SPIM-FCS) using a position-sensitive detector

15:20      Malte Wachsmuth (EMBL, Heidelberg, D): Fluorescence correlation spectroscopy with higher throughput


15:50 – 16:10     Coffee break


Session 4

16:10      Christian Eggeling(MPI BPC, Göttingen, D): Fluorescence Correlation Spectroscopy at the nanoscale: The Combination with STED microscopy

16:40      Carlo Manzo(Institut de Ciencies Fotoniques, E): Nanoscale fluorescence correlation spectroscopy on intact living cell membranes with NSOM probes

17:10      Ralf Kühnemuth(U. Düsseldorf, D): Multiparameter Fluorescence Image Spectroscopy

17:10     Closing

19:00      Farewell Dinner (OEC Hostel III Dining Hall)



  1. Acceptor photobleaching and ratiometric FRET microscopy. Tutors: György Vereb - László Újlaky-Nagy -Dániel Beyer - Árpád Szöőr - Tamás Lajtos.Zeiss LSM 510

Interaction of transcription factors Fos-CFP and Jun-YFP

  1. Flow cytometric FRET. Tutors: Andrea Bodnár – Attila Forgács – Enikő Nizsalóczki. BD FACSARIA

Interaction of cell surface proteins

  1. FCS. Tutors: György Vámosi – Jörg Langowski – Thomas Weidemann – Gábor Mocsár. Olympus FV1000-FCS at Theor. Bldg.

Diffusion of nuclear receptors RAR-GFP

  1. Number and brightness analysis. Tutors: Péter Nagy – Timea Váradi - Tamás Kovács. Olympus FV1000 at Dept. of Anatomy

Stoichiometry of association of erbB receptors

  1. FRAP. Tutors: Rainer Pepperkok – Stefan Terjung – Péter Brázda. Zeiss LSM 510 at Dept. of Physiology

Diffusion of EGF receptors or RAR-GFP nuclear receptors

FRAP_tutorial_EMBO_Debrecen.pdf2.67 MB
N&B+coloc_EMBO2011Debr_PeterNagy.pdf2.28 MB

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