Program
Aug 16, Tuesday
10:00 – 16:00 Registration (Lobby of Life Science Building)
12:30 – 13:45 Lunch (OEC Hostel III Dining Hall)
14:00 Opening by Sándor Damjanovich and György Vámosi(LSB F008-009)
14:20 – 15:10 Robert M. Clegg (U. Illinois, Urbana-Champaign, US): The life in a normal day (nanoseconds) of an excited fluorescent molecule
15:20 – 16:10 György Vereb (U. Debrecen, H): Don’t fret over FRET
16:20 – 16:40Coffee break
16:40 – 17:30 Jörg Langowski (DKFZ, Heidelberg, D): FCS and FCCS – introduction and applications in biology
/sites/default/files/FCS%20intro.pdf
17:40 – 18:30 Péter Nagy (U. Debrecen, H): Molecular interactions investigated by quantitative colocalization measurements and number & brightness analysis http://embo2011.unideb.hu/sites/default/files/N&B+coloc_EMBO2011Debr_PeterNagy_0.pdf18:40
– 19:30 Stefan Terjung (EMBL, Heidelberg, D): FRAP tutorial
http://embo2011.unideb.hu/sites/default/files/FRAP_tutorial_EMBO_Debrecen.pdf
19:30 Dinner (LSB lobby)
Aug 17, Wednesday
9:00 – 12:30 Practice
12:30 – 13:45 Lunch (OEC Hostel III Dining Hall)
14:00 – 16:30 Evaluation
16:30 – 17:00 Coffee Break
17:00 Katalin Tóth (DKFZ, Heidelberg, D): Structure, stability and diversity of nucleosomes studied by single pair FRET
17:40 György Vámosi (U. Debrecen, H): Interactions and function of membrane receptors and transcription factors studied by FRET and FCS
18:20 Robert M. Clegg (U. Illinois, Urbana-Champaign, US): Chasing Fluorescence Lifetimes in Complex Biological Systems. What Can Fluorescence Lifetime Imaging Microscopy (FLIM) tell us?
19:30 Dinner (OEC Hostel III Dining Hall)
Aug 18, Thursday
9:00 – 12:30 Practice
12:30 – 13:45 Lunch (OEC Hostel III Dining Hall)
14:00 – 16:30 Evaluation
16:30 – 17:00 Coffee Break
17:00 Rainer Pepperkok (EMBL, Heidelberg, D): Studying the Coupling of COPII Vesicle Formation to Microtubule-Mediated Transport by FRAP and Mathematcial Modelling
17:40 Claus Seidel (U. Düsseldorf, D): Multiparameter Fluorescence Image Spectroscopy (MFIS) to study molecular interactions I
18:45 Poster viewing with wine and cheese, 20:00- dinner (LSB lobby)
Aug 19, Friday
9:00 – 12:30 Practice
12:30 – 13:45 Lunch (OEC Hostel III Dining Hall)
14:00 – 17:00 Practice
17:00 – 17:30Coffee Break
17:30 Claus Seidel (U. Düsseldorf, D): Multiparameter Fluorescence Image Spectroscopy (MFIS) to study molecular interactions II
18:10 Jerker Widengren (KTH Stockholm, S): Fast molecular dynamic processes monitored by fluorescence correlation spectroscopy
19:30 Dinner at Paripa Csárda (buses leave from Life Science Building)
Aug 20, Saturday
Viewing Flower Carnival (from the bleachers at Egyetem sugárút)
13:00 – 14:00 Lunch (OEC Hostel III Dining Hall)
14:30 - 16:30 Evaluation of Aug 19 morning practice
16:30 – 17:00Coffee break
17:00 - 19:00 Evaluation of Aug 19 afternoon practice
19:30 Dinner (OEC Hostel III Dining Hall)
Aug 21, Sunday
9:00 – 12:30 Practice
12:30 – 13:30 Lunch (OEC Hostel III Dining Hall)
13:30 – 15:30 Evaluation
16:00 – 17:00 Discussion of the labs presented by participants
17:00 – 17:30 Coffee break
17:30 Giuseppe Chirico (U. Milan, I): Fluorescence Correlation Spectroscopy methods for in vitro and in vivo experiments
18:10 János Szöllősi (U. Debrecen, H): Novel FRET approaches for studying interactions of membrane molecules
18:50 Malte Renz (NIH, Bethesda, US): Single-Molecule Counting with PALM
19:50 Dinner (OEC Hostel III Dining Hall)
Aug 22, Monday, EBSA Satellite Meeting on “FCS in Cell Biology”
Session 1
9:00 Opening
9:10 Yves Engelborghs (U. Leuven, B): Protein diffusion and interactions in vitro and in the living cell as studied by Fluorescence Correlation Spectroscopy
9:40 Roland Brock(U. Nijmegen, NL): Quantitative Analysis of Signaling Proteins and Complex Formation in T Cell Signal Transduction
10:10 Thomas Weidemann(U. Dresden, D): Advanced concepts of fluorescence correlation spectroscopy to study molecular interactions in cytokine receptor signaling
10:40 - 11.00 Coffee break
Session 2
11:00 Jerker Widengren(KTH, Stockholm, S): Transient state monitoring – approaches and possible benefits for biomolecular studies
11:30 Markus Sauer(U. Würzburg, D): Super-Resolution Imaging by dSTORM
12:00 Giuseppe Chirico (U. Milan, I): Diffusion-Photodynamics coupling in FCS studies of photo-switchable GFP proteins
12.30-13:30 Lunch (LSB Lobby)
13:30-14:50 Poster session (LSB Lobby)
Session 3
14:50 Jörg Langowski(DKFZ, Heidelberg, D): Single Plane Illumination Fluorescence Correlation Spectroscopy (SPIM-FCS) using a position-sensitive detector
15:20 Malte Wachsmuth (EMBL, Heidelberg, D): Fluorescence correlation spectroscopy with higher throughput
15:50 – 16:10 Coffee break
Session 4
16:10 Christian Eggeling(MPI BPC, Göttingen, D): Fluorescence Correlation Spectroscopy at the nanoscale: The Combination with STED microscopy
16:40 Carlo Manzo(Institut de Ciencies Fotoniques, E): Nanoscale fluorescence correlation spectroscopy on intact living cell membranes with NSOM probes
17:10 Ralf Kühnemuth(U. Düsseldorf, D): Multiparameter Fluorescence Image Spectroscopy
17:10 Closing
19:00 Farewell Dinner (OEC Hostel III Dining Hall)
Practices:
- Acceptor photobleaching and ratiometric FRET microscopy. Tutors: György Vereb - László Újlaky-Nagy -Dániel Beyer - Árpád Szöőr - Tamás Lajtos.Zeiss LSM 510
Interaction of transcription factors Fos-CFP and Jun-YFP
- Flow cytometric FRET. Tutors: Andrea Bodnár – Attila Forgács – Enikő Nizsalóczki. BD FACSARIA
Interaction of cell surface proteins
- FCS. Tutors: György Vámosi – Jörg Langowski – Thomas Weidemann – Gábor Mocsár. Olympus FV1000-FCS at Theor. Bldg.
Diffusion of nuclear receptors RAR-GFP
- Number and brightness analysis. Tutors: Péter Nagy – Timea Váradi - Tamás Kovács. Olympus FV1000 at Dept. of Anatomy
Stoichiometry of association of erbB receptors
- FRAP. Tutors: Rainer Pepperkok – Stefan Terjung – Péter Brázda. Zeiss LSM 510 at Dept. of Physiology
Diffusion of EGF receptors or RAR-GFP nuclear receptors
| Attachment | Size |
|---|---|
| FRAP_tutorial_EMBO_Debrecen.pdf | 2.67 MB |
| N&B+coloc_EMBO2011Debr_PeterNagy.pdf | 2.28 MB |
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